PDF DatasheetHuman PLA1A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PLA1A based upon sequence from Human
Host: QP13070 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PLA1A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13070.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSDAPPTPQ PKCADFQSAN LFEGTDLKVQ FLLFVPSNPS CGQLVEGSSD LQNSGFNATL GTKLIIHGFR VLGTKPSWID TFIRTLLRAT NANVIAVDWI YGSTGVYFSA VKNVIKLSLE ISLFLNKLLV LGVSESSIHI IGVSLGAHVG GMVGQLFGGQ LGQITGLDPA GPEYTRASVE ERLDAGDALF VEAIHTDTDN LGIRIPVGHV DYFVNGGQDQ PGCPTFFYAG YSYLICDHMR AVHLYISALE NSCPLMAFPC ASYKAFLAGR CLDCFNPFLL SCPRIGLVEQ GGVKIEPLPK EVKVYLLTTS SAPYCMHHSL VEFHLKELRN KDTNIEVTFL SSNITSSSKI TIPKQQRYGK GIIAHATPQC QINQVKFKFQ SSNRVWKKDR TTIIGKFCTA LLPVNDREKM VCLPEPVNLQ ASVTVSCDLK IACV
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The PLA1A solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human PLA1A General Information | |
|---|---|
| Alternate Names | |
| Phospholipase A1 Member A, PSPLA1, PS-PLA1, Phosphatidylserine-Specific Phospholipase A1alpha, EC 3.1. 1. -, NMD, Phosphatidylserine-Specific Phospholipase A1, PLA1A. | |
| Molecular Weight | |
| 49.5 kDa | |
| Chromosomal Location | |
| q13.33 on chromosome 3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PLA1A |
| Entrez Gene ID | 51365 |
| UniProt ID(s) | Q53H76 |
| COSMIC ID Link(s) | PLA1A |
| KEGG Gene ID(s) | hsa:51365 |
| General Description of Recombinant Human PLA1A. | |
| Human PLA1A produced in E. coli is a single, non-glycosylated polypeptide chain containing 454 amino acids (26-456) and having a molecular mass of 49.5kDa. PLA1A is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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