PDF DatasheetHuman PNPT1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PNPT1 based upon sequence from Human
Host: QP13086 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PNPT1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13086.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSAVAVDLG NRKLEISSGK LARFADGSAV VQSGDTAVMV TAVSKTKPSP SQFMPLVVDY RQKAAAAGRI PTNYLRREIG TSDKEILTSR IIDRSIRPLF PAGYFYDTQV LCNLLAVDGV NEPDVLAING ASVALSLSDI PWNGPVGAVR IGIIDGEYVV NPTRKEMSSS TLNLVVAGAP KSQIVMLEAS AENILQQDFC HAIKVGVKYT QQIIQGIQQL VKETGVTKRT PQKLFTPSPE IVKYTHKLAM ERLYAVFTDY EHDKVSRDEA VNKIRLDTEE QLKEKFPEAD PYEIIESFNV VAKEVFRSIV LNEYKRCDGR DLTSLRNVSC EVDMFKTLHG SALFQRGQTQ VLCTVTFDSL ESGIKSDQVI TAINGIKDKN FMLHYEFPPY ATNEIGKVTG LNRRELGHGA LAEKALYPVI PRDFPFTIRV TSEVLESNGS SSMASACGGS LALMDSGVPI SSAVAGVAIG LVTKTDPEKG EIEDYRLLTD ILGIEDYNGD MDFKIAGTNK GITALQADIK LPGIPIKIVM EAIQQASVAK KEILQIMNKT ISKPRASRKE NGPVVETVQV PLSKRAKFVG PGGYNLKKLQ AETGVTISQV DEETFSVFAP TPSAMHEARD FITEICKDDQ EQQLEFGAVY TATITEIRDT GVMVKLYPNM TAVLLHNTQL DQRKIKHPTA LGLEVGQEIQ VKYFGRDPAD GRMRLSRKVL QSPATTVVRT LNDRSSIVMG EPISQSSSNS Q
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PNPT1 protein solution (0.25 mg/ml) containing Phosphate buffered saline (pH 7.4), 10% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human PNPT1 General Information | |
|---|---|
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| Alternate Names | |
| Polyribonucleotide Nucleotidyltransferase 1, Polynucleotide Phosphorylase-Like Protein, Polynucleotide Phosphorylase 1, 3-5 RNA Exonuclease OLD35, PNPase Old-35, EC 2.7. 7.8, PNPase 1, COXPD13, DFNB70, PNPASE, OLD35, Polyribonucleotide Nucleotidyltransferase 1, Mitochondrial, Deafness, Autosomal Recessive 70, Polynucleotide Phosphorylase, 3-5 RNA Exonuclease, EC 2.7. 7, Old-35, Polyribonucleotide nucleotidyltransferase 1, mitochondrial, 3′-5′ RNA exonuclease OLD35, PNPase old-35. | |
| Molecular Weight | |
| 83.3 kDa | |
| Chromosomal Location | |
| p16.1 on chromosome 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PNPT1 |
| Entrez Gene ID | 87178 |
| UniProt ID(s) | Q8TCS8 |
| COSMIC ID Link(s) | PNPT1 |
| KEGG Gene ID(s) | hsa:87178 |
| General Description of Recombinant Human PNPT1. | |
| Human PNPT1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 761 amino acids (46-783 AA) and having a molecular mass of 83.3kDa. PNPT1 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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