PDF DatasheetHuman POLB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant POLB based upon sequence from Human
Host: QP13091 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of POLB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13091.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSE
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: POLB protein solution (0.5 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol and 0.1M NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human POLB General Information | |
|---|---|
 |
|
| Alternate Names | |
| DNA polymerase beta, POLB. | |
| Molecular Weight | |
| 40.3 kDa | |
| Chromosomal Location | |
| p11.21 on chromosome 8 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | POLB |
| Entrez Gene ID | 5423 |
| UniProt ID(s) | P06746 |
| COSMIC ID Link(s) | POLB |
| KEGG Gene ID(s) | hsa:5423 |
| General Description of Recombinant Human POLB. | |
| Human POLB produced in E. Coli is a single, non-glycosylated polypeptide chain containing 355 amino acids (1-335 AA) and having a molecular mass of 40.3kDa. POLB is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.