PDF DatasheetHuman POLR2J2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant POLR2J2 based upon sequence from Human
Host: QP13103 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of POLR2J2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13103.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP KACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The POLR2J2 solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol, 1mM DTT and 250mM Imidazole.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human POLR2J2 General Information | |
|---|---|
| Alternate Names | |
| HRPB11B, RPB11b1, POLR2J2, DNA-directed RNA polymerase II subunit RPB11-b1. | |
| Molecular Weight | |
| 15.5 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | F6W009 |
| General Description of Recombinant Human POLR2J2. | |
| Human POLR2J2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 138 amino acids (1-115) and having a molecular mass of 15.5kDa. POLR2J2 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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