PDF DatasheetHuman POLR2K Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant POLR2K based upon sequence from Human
Host: QP13105 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of POLR2K was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13105.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDTQKDV QPPKQQPMIY ICGECHTENE IKSRDPIRCR ECGYRIMYKK RTKRLVVFDA R
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: POLR2K protein solution (0.5 mg/ml) containing Phosphate buffered saline (pH 7.4), 10% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human POLR2K General Information | |
|---|---|
| Alternate Names | |
| Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa, RNA Polymerases I, II, And III Subunit ABC4, DNA-Directed RNA Polymerase II Subunit K, RNA Polymerase II 7.0 KDa Subunit, ABC10-Alpha, RPB10alpha, RPB7.0, RPB12,DNA Directed RNA Polymerases I, II, And III 7.0 Kda Polypeptide, DNA-Directed RNA Polymerases I, II, And III Subunit RPABC4, Polymerase (RNA) II (DNA Directed) Polypeptide K (7.0kD), HsRPB10a, HRPB7.0, RPABC4, POLR2K. | |
| Molecular Weight | |
| 9.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P53803 |
| General Description of Recombinant Human POLR2K. | |
| Human POLR2K produced in E. Coli is a single, non-glycosylated polypeptide chain containing 81 amino acids (1-58 AA) and having a molecular mass of 9.4kDa. POLR2K is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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