PDF DatasheetHuman POLR3F Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant POLR3F based upon sequence from Human
Host: QP13106 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of POLR3F was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13106.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEVKVK VQPPDADPVE IENRIIELCH QFPHGITDQV IQNEMPHIEA QQRAVAINRL LSMGQLDLLR SNTGLLYRIK DSQNAGKMKG SDNQEKLVYQ IIEDAGNKGI WSRDIRYKSN LPLTEINKIL KNLESKKLIK AVKSVAASKK KVYMLYNLQP DRSVTGGAWY SDQDFESEFV EVLNQQCFKF LQSKAETARE SKQNPMIQRN SSFASSHEVW KYICELGISK VELSMEDIET ILNTLIYDGK VEMTIIAAKE GTVGSVDGHM KLYRAVNPII PPTGLVRAPC GLCPVFDDCH EGGEISPSNC IYMTEWLEF
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The POLR3F solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 30% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human POLR3F General Information | |
|---|---|
| Alternate Names | |
| DNA-directed RNA polymerase III subunit RPC6, RNA polymerase III subunit C6, DNA-directed RNA polymerase III subunit F, RNA polymerase III 39 kDa subunit, RPC39, POLR3F, RPC6. | |
| Molecular Weight | |
| 38.1 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9H1D9 |
| General Description of Recombinant Human POLR3F. | |
| Human POLR3F produced in E. coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-316) and having a molecular mass of 38.1kDa. POLR3F is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.