PDF DatasheetHuman POU6F1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant POU6F1 based upon sequence from Human
Host: QP13111 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of POU6F1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13111.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPGISSQ ILTNAQGQVI GTLPWVVNSA SVAAPAPAQS LQVQAVTPQL LLNAQGQVIA TLASSPLPPP VAVRKPSTPE SPAKSEVQPI QPTPTVPQPA VVIASPAPAA KPSASAPIPI TCSETPTVSQ LVSKPHTPSL DEDGINLEEI REFAKNFKIR RLSLGLTQTQ VGQALTATEG PAYSQSAICR FEKLDITPKS AQKLKPVLEK WLNEAELRNQ EGQQNLMEFV GGEPSKKRKR RTSFTPQAIE ALNAYFEKNP LPTGQEITEI AKELNYDREV VRVWFCNRRQ TLKNTSKLNV FQIP
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The POU5F1 solution contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human POU6F1 General Information | |
|---|---|
| Alternate Names | |
| BRN5, MPOU, TCFB1, Brain-5, Brn-5, mPOU homeobox protein, Brain-specific homeobox/POU domain protein 5. | |
| Molecular Weight | |
| 35 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q14863 |
| General Description of Recombinant Human POU6F1. | |
| Human POU6F1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 324 amino acids (1-301 AA ) and having a molecular mass of 35kDa. POU6F1 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.