PDF DatasheetHuman PPP3CA Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PPP3CA based upon sequence from Human
Host: QP13128 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PPP3CA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13128.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH TGSMSEPKAI DPKLSTTDRV VKAVPFPPSH RLTAKEVFDN DGKPRVDILK AHLMKEGRLE ESVALRIITE GASILRQEKN LLDIDAPVTV CGDIHGQFFD LMKLFEVGGS PANTRYLFLG DYVDRGYFSI ECVLYLWALK ILYPKTLFLL RGNHECRHLT EYFTFKQECK IKYSERVYDA CMDAFDCLPL AALMNQQFLC VHGGLSPEIN TLDDIRKLDR FKEPPAYGPM CDILWSDPLE DFGNEKTQEH FTHNTVRGCS YFYSYPAVCE FLQHNNLLSI LRAHEAQDAG YRMYRKSQTT GFPSLITIFS APNYLDVYNN KAAVLKYENN VMNIRQFNCS PHPYWLPNFM DVFTWSLPFV GEKVTEMLVN VLNICSDDEL GSEEDGFDGA TAAARKEVIR NKIRAIGKMA RVFSVLREES ESVLTLKGLT PTGMLPSGVL SGGKQTLQSA IKGFSPQHKI TSFEEAKGLD RINERMPPRR DAMPSDANLN SINKALTSET NGTDSNGSNS SNIQ
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The PPP3CA solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human PPP3CA General Information | |
|---|---|
| Alternate Names | |
| Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit alpha isoform, PPP3CA, CALNA, CAN, CALN, CCN1, CNA1, PPP2B, CALNA1. | |
| Molecular Weight | |
| 60 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q08209 |
| General Description of Recombinant Human PPP3CA. | |
| Human PPP3CA fused with a 23 amino acid His tag at N-terminus produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 534 amino acids (1-511 AA ) and having a molecular mass of 60kDa. The PPP3CA is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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