PDF Datasheetother Prelamin-A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Prelamin-A based upon sequence from other
Host: QP13135 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Prelamin-A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13135.
Bioactivity Data: Untested
Amino Acid Sequence: metpsqrrat rsgaqasstp lsptritrlq ekedlqelnd rlavyidrvr sletenaglr lriteseevv srevsgikaa yeaelgdark tldsvakera rlqlelskvr eefkelkarn tkkegdliaa qarlkdleal lnskeaalst alsekrtleg elhdlrgqva kleaalgeak kqlqdemlrr vdaenrlqtm keeldfqkni yseelretkr rhetrlveid ngkqrefesr ladalqelra qhedqveqyk kelektysak ldnarqsaer nsnlvgaahe elqqsririd slsaqlsqlq kqlaakeakl rdledslare rdtsrrllae keremaemra rmqqqldeyq elldiklald meihayrkll egeeerlrls psptsqrsrg rasshssqtq gggsvtkkrk lestesrssf sqhartsgrv aveevdeegk fvrlrnksne dqsmgnwqik rqngddpllt yrfppkftlk agqvvtiwaa gagathsppt dlvwkaqntw gcgnslrtal instgeevam rklvrsvtvv eddededgdd llhhhhgshc sssgdpaeyn lrsrtvlcgt cgqpadkasa sgsgaqvggp issgssassv tvtrsyrsvg gsgggsfgdn lvtrsyllgn ssprtqspqn csim
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The Prelamin-A solution (0.1 mg/ml) contains 10% Glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant other Prelamin-A General Information | |
|---|---|
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| Alternate Names | |
| Prelamin-A/C, LMNA, LMN1, FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMNC, PRO1, CDCD1, CMD1A, FPLD2, LMNL1, CMT2B1, LGMD1B. | |
| Molecular Weight | |
| 74.1 kDa | |
| Chromosomal Location | |
| q22 on chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LMNA |
| Entrez Gene ID | 4000 |
| UniProt ID(s) | P02545 |
| COSMIC ID Link(s) | LMNA |
| KEGG Gene ID(s) | hsa:4000 |
| General Description of Recombinant other Prelamin-A. | |
| Human Recombinant Prelamin-A is a 74kDa precursor of the nuclear lamin A protein. Prelamin-A is a structural component of the nuclear lamina and it is encoded by lamin A/C gene (LMNA). Due to the presence of a CAAX box sequence at carboxyl terminus, Prelamin-A in vivo goes through a serial of post-translational modifications, resulting in the farnesylation of the cysteine thiol, removal of the AAX tripeptide, carboxyl-methylation of the cysteinyl carboxy group and proteolysis of 18 C-terminal amino acids residues that lead to mature lamin A. Diverse mutations in the lamin A/C gene are associated with different diseases that are collectively called laminopathies, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. prelamin A is fused to a 6 Histidine tag at the N-terminus. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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