PDF DatasheetHuman PTEN Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PTEN based upon sequence from Human
Host: QP13199 protein expressed in E. Coli.
Available Tags: His, GST
Protein Construction: A cDNA sequence encoding the sequence of PTEN was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13199.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTAIIKEIVS RNKRRYQEDG FDLDLTYIYP NIIAMGFPAE RLEGVYRNNI DDVVRFLDSK HKNHYKIYNL CAERHYDTAK FNCRVAQYPF EDHNPPQLEL IKPFCEDLDQ WLSEDDNHVA AIHCKAGKGR TGVMICAYLL HRGKFLKAQE ALDFYGEVRT RDKKGVTIPS QRRYVYYYSY LLKNHLDYRP VALLFHKMMF ETIPMFSGGT CNPQFVVCQL KVKIYSSNSG PTRREDKFMY FEFPQPLPVC GDIKVEFFHK QNKMLKKDKM FHFWVNTFFI PGPEETSEKV ENGSLCDQEI DSICSIERAD NDKEYLVLTL TKNDLDKANK DKANRYFSPN FKVKLYFTKT VEEPSNPEAS SSTSVTPDVS DNEPDHYRYS DTTDSDPENE PFDEDQHTQI TKV
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The PTEN solution contains 20mM Tris-HCl buffer (pH 8.0), 1mM EDTA, 2mM DTT, 100mM NaCl, and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human PTEN General Information | |
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| Alternate Names | |
| Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, EC 3.1. 3.67, EC 3.1. 3.16, EC 3.1. 3.48, Phosphatase and tensin homolog, Mutated in multiple advanced cancers 1, PTEN, MMAC1, TEP1, BZS, MHAM, PTEN1, 10q23del, MGC11227. | |
| Molecular Weight | |
| 49.3 kDa | |
| Chromosomal Location | |
| q23.31 on chromosome 10 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PTEN |
| Entrez Gene ID | 5728 |
| UniProt ID(s) | P60484 |
| COSMIC ID Link(s) | PTEN |
| KEGG Gene ID(s) | hsa:5728 |
| General Description of Recombinant Human PTEN. | |
| Human PTEN fused with a 20 amino acid His tag at N-terminus produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 423 amino acids (1.403 AA ) and having a molecular mass of 49.3kDa. The PTEN is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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