PDF DatasheetHuman RAP1B Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant RAP1B based upon sequence from Human
Host: QP13266 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of RAP1B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13266.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD LVRQINRKTP VPGKARKKSS C
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The RAP1B protein solution (0.25 mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 5mM DTT and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human RAP1B General Information | |
|---|---|
| Alternate Names | |
| RAP1B, member of RAS oncogene family, DKFZp586H0723, K-REV, RAL1B, GTP-binding protein smg p21B, Ras family small GTP binding protein RAP1B, RAS-related protein RAP1B, small GTP binding protein. | |
| Molecular Weight | |
| 22.6 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P61224 |
| General Description of Recombinant Human RAP1B. | |
| RAP1B produced in E. Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181. AA) and having a molecular mass of 22.6kDa. RAP1B is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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