PDF DatasheetHuman RDBP Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant RDBP based upon sequence from Human
Host: QP13291 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of RDBP was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13291.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE KGPVPTFQPF QRSISADDDL QESSRRPQRK SLYESFVSSS DRLRELGPDG EEAEGPGAGD GPPRSFDWGY EERSGAHSSA SPPRSRSRDR SHERNRDRDR DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR DREGPFRRSD SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV QNSPKGCHRD KRTQIVYSDD VYKENLVDGF
Purity: RDBP purity was found to be greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The RDBP protein solution (1 mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 2mM DTT and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: The RDBP is supplied as a sterile filtered clear solution.
| Recombinant Human RDBP General Information | |
|---|---|
| Alternate Names | |
| RD RNA binding protein, NELF-E, RD, D6S45, RDP, Major Histocompatibility Complex Gene RD, Negative Elongation Factor Polypeptide E, nuclear protein, RDBP. | |
| Molecular Weight | |
| 45.4 kDa | |
| Chromosomal Location | |
| on chromosome CHR_HSCHR6_MHC_SSTO_CTG1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NELFE |
| Entrez Gene ID | 7936 |
| UniProt ID(s) | P18615 |
| COSMIC ID Link(s) | NELFE |
| KEGG Gene ID(s) | hsa:7936 |
| General Description of Recombinant Human RDBP. | |
| RDBP produced in E. Coli is a single, non-glycosylated polypeptide chain containing 400 amino acids (1-380AA ) and having a molecular mass of 45.4 kDa. RDBP is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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