PDF DatasheetHuman RHEB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant RHEB based upon sequence from Human
Host: QP13313 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of RHEB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13313.
Bioactivity Data: Untested
Amino Acid Sequence: MASMTGGQQM GRGSASMPQS KSRKIAILGY RSVGKSSLTI QFVEGQFVDS YDPTIENTFT KLITVNGQEY HLQLVDTAGQ DEYSIFPQTY SIDINGYILV YSVTSIKSFE VIKVIHGKLL DMVGKVQIPI MLVGNKKDLH MERVISYEEG KALAESWNAA FLESSAKENQ TAVDVFRRII LEAEKMDGAA SQGKSSC
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The RHEB protein solution (1 mg/ml) contains 20mM Tris-HCl pH 8.0, 1mM DTT and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human RHEB General Information | |
|---|---|
| Alternate Names | |
| RHEB2, GTP-binding protein Rheb, MGC111559, Ras homolog enriched in brain, RHEB. | |
| Molecular Weight | |
| 21.7 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q15382 |
| General Description of Recombinant Human RHEB. | |
| Human RHEB produced in E. Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids (1-181 amino acids) and having a molecular mass of 21.7 kDa. The RHEB is fused to T7-tag at N-terminus (16 AA ) and is purified by standard chromatography techniques. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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