PDF DatasheetHuman RNASE3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant RNASE3 based upon sequence from Human
Host: QP13323 protein expressed in Insect, E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of RNASE3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13323.
Bioactivity Data: Untested
Amino Acid Sequence: ADPRPPQFTR AQWFAIQHIS LNPPRCTIAM RAINNYRWRC KNQNTFLRTT FANVVNVCGN QSIRCPHNRT LNNCHRSRFR VPLLHCDLIN PGAQNISNCR YADRPGRRFY VVACDNRDPR DSPRYPVVPV HLDTTIHHHH HH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: RNASE3 protein solution (0.5 mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human RNASE3 General Information | |
|---|---|
| Alternate Names | |
| ECP, RNS3, Ribonuclease 3, Eosinophil cationic protein, RNASE3, RNASE3. | |
| Molecular Weight | |
| 16.6 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P12724 |
| General Description of Recombinant Human RNASE3. | |
| Human RNASE3 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 142 amino acids (28-160 AA ) and having a molecular mass of 16.6kDa (Migrates at 18-28kDa on SDS-PAGE under reducing conditions). RNASE3 is expressed with a 6 amino acid His tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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