PDF DatasheetHuman SDHAF1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant SDHAF1 based upon sequence from Human
Host: QP13441 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of SDHAF1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13441.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSRHSRL QRQVLSLYRD LLRAGRGKPG AEARVRAEFR QHAGLPRSDV LRIEYLYRRG RRQLQLLRSG HATAMGAFVR PRAPTGEPGG VGSQPDDGDS PRNPHDSTGA PETRPDGR
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: SDHAF1 protein solution (0.25 mg/ml) containing Phosphate buffered saline (pH 7.4), 30% glycerol, 2mM DTT and 0.1mM PMSF.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human SDHAF1 General Information | |
|---|---|
| Alternate Names | |
| Succinate Dehydrogenase Complex Assembly Factor 1, LYR Motif-Containing Protein 8, LYR Motif Containing 8, SDH Assembly Factor 1, LYRM8, Succinate Dehydrogenase Assembly Factor 1, Mitochondrial, Succinate dehydrogenase assembly factor 1, mitochondrial. | |
| Molecular Weight | |
| 15.2 kDa | |
| Chromosomal Location | |
| q13.12 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SDHAF1 |
| Entrez Gene ID | 644096 |
| UniProt ID(s) | A6NFY7 |
| COSMIC ID Link(s) | SDHAF1 |
| KEGG Gene ID(s) | hsa:644096 |
| General Description of Recombinant Human SDHAF1. | |
| Human SDHAF1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 138 amino acids (1-115 AA) and having a molecular mass of 15.2kDa. SDHAF1 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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