PDF DatasheetHuman SEC61B Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant SEC61B based upon sequence from Human
Host: QP13447 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of SEC61B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13447.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPGPTPS GTNVGSSGRS PSKAVAARAA GSTVRQRKNA SCGTRSAGRT TSAGTGGMWR FYTEDSPGLK VGP
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: SEC61B protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human SEC61B General Information | |
|---|---|
| Alternate Names | |
| Protein transport protein Sec61 subunit beta, SEC61B, Sec61 Beta Subunit, Protein Translocation Complex Beta, Protein Transport Protein SEC61 Beta Subunit, Sec61 Complex Beta Subunit. | |
| Molecular Weight | |
| 9.4 kDa | |
| Chromosomal Location | |
| q22.33 on chromosome 9 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SEC61B |
| Entrez Gene ID | 10952 |
| UniProt ID(s) | P60468 |
| COSMIC ID Link(s) | SEC61B |
| KEGG Gene ID(s) | hsa:10952 |
| General Description of Recombinant Human SEC61B. | |
| Human SEC61B produced in E. Coli is a single, non-glycosylated polypeptide chain containing 93 amino acids (1-70 AA) and having a molecular mass of 9.4kDa. SEC61B is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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