PDF DatasheetHuman SIKE1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant SIKE1 based upon sequence from Human
Host: QP13502 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of SIKE1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13502.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSCTIEK ILTDAKTLLE RLREHDAAAE SLVDQSAALH RRVAAMREAG TALPDQYQED ASDMKDMSKY KPHILLSQEN TQIRDLQQEN RELWISLEEH QDALELIMSK YRKQMLQLMV AKKAVDAEPV LKAHQSHSAE IESQIDRICE MGEVMRKAVQ VDDDQFCKIQ EKLAQLELEN KELRELLSIS SESLQARKEN SMDTASQAIK
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: SIKE1 protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human SIKE1 General Information | |
|---|---|
| Alternate Names | |
| Suppressor Of IKBKE 1, SIKE, Suppressor Of IKK Epsilon, Suppressor Of IKK-Epsilon. | |
| Molecular Weight | |
| 26.1 kDa | |
| Chromosomal Location | |
| p13.2 on chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SIKE1 |
| Entrez Gene ID | 80143 |
| UniProt ID(s) | Q9BRV8 |
| COSMIC ID Link(s) | SIKE1 |
| KEGG Gene ID(s) | hsa:80143 |
| General Description of Recombinant Human SIKE1. | |
| Human SIKE1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 230 amino acids (1-207 AA) and having a molecular mass of 26.1kDa. SIKE1 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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