PDF DatasheetHuman SIRT5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant SIRT5 based upon sequence from Human
Host: QP13510 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of SIRT5 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13510.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSARPSSSM ADFRKFFAKA KHIVIISGAG VSAESGVPTF RGAGGYWRKW QAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAG TKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQ GPCGTTLPEA LACHENETVS
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: SIRT5 protein solution (0.5 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 30% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human SIRT5 General Information | |
|---|---|
| Alternate Names | |
| Sirtuin 5, NAD-Dependent Lysine Demalonylase And Desuccinylase Sirtuin-5,Mitochondria, NAD-Dependent Protein Deacylase Sirtuin-5 Mitochondrial, Silent Mating Type Information Regulation 2 (S. Cerevisiae) Homolog 5, NAD-Dependent Deacetylase Sirtuin-5, Regulatory Protein SIR2 Homolog 5, SIR2-Like Protein 5, SIR2L5, Sirtuin Type 5, Sir2-Like 5, EC 3.5. 1. -. | |
| Molecular Weight | |
| 32.5 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9NXA8 |
| General Description of Recombinant Human SIRT5. | |
| Human SIRT5 produced in E. Coli is a single, non-glycosylated polypeptide chain topological domain containing 300 amino acids (34-310 AA) and having a molecular mass of 32.5kDa. SIRT5 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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