PDF DatasheetHuman SIT1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant SIT1 based upon sequence from Human
Host: QP13511 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of SIT1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13511.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MHLSQWTRGR SRSHPGQGRS GESVEEVPLY GNLHYLQTGR LSQDPEPDQQ DPTLGGPARA AEEVMCYTSL QLRPPQGRIP GPGTPVKYSE VVLDSEPKSQ ASGPEPELYA SVCAQTRRAR ASFPDQAYAN SQPAAS
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: SIT1 protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human SIT1 General Information | |
|---|---|
| Alternate Names | |
| Signaling threshold-regulating transmembrane adapter 1, SHP2-interacting transmembrane adapter protein, Suppression-inducing transmembrane adapter 1, gp30/40, SIT1, SIT, RP11-331F9.5, MGC125908, MGC125909, MGC125910. | |
| Molecular Weight | |
| 16.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9Y3P8 |
| General Description of Recombinant Human SIT1. | |
| Human SIT1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (62-196 AA ) and having a molecular mass of 16.9kDa (Molecular weight on SDS-PAGE will appear higher). SIT1 is fused to a 21 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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