PDF DatasheetHuman SPRED1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant SPRED1 based upon sequence from Human
Host: QP13583 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of SPRED1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13583.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSEETAT SDNDNSYARV RAVVMTRDDS SGGWLPLGGS GLSSVTVFKV PHQEENGCAD FFIRGERLRD KMVVLECMLK KDLIYNKVTP TFHHWKIDDK KFGLTFQSPA DARAFDRGIR RAIEDISQGC PESKNEAEGA DDLQANEEDS SSSLVKDHLF QQETVVTSEP YRSSNIRPSP FEDLNARRVY MQSQANQITF GQPGLDIQSR SMEYVQRQIS KECGSLKSQN RVPLKSIRHV SFQDEDEIVR INPRDILIRR YADYRHPDMW KNDLERDDAD SSIQFSKPDS KKSDYLYSCG DETKLSSPKD SVVFKTQPSS LKIKKSKRRK EDGERSRCVY CQERFNHEEN VRGKCQDAPD PIKRCIYQVS CMLCAESMLY HCMSDSEGDF SDPCSCDTSD DKFCLRWLAL VALSFIVPCM CCYVPLRMCH RCGEACGCCG GKHKAAG
Purity: Greater than 80.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The SPRED1 solution (0.5 mg/1ml) contains 20mM Tris-HCl (pH 8.0) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human SPRED1 General Information | |
|---|---|
| Alternate Names | |
| Sprouty-Related EVH1 Domain Containing 1, SPRED1, Spred-1, hSpred1, NFLS. | |
| Molecular Weight | |
| 52.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q7Z699 |
| General Description of Recombinant Human SPRED1. | |
| Human SPRED1 produced in E. coli is a single polypeptide chain containing 467 amino acids (1-444) and having a molecular mass of 52.9kDa. SPRED1 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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