PDF DatasheetHuman TK2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant TK2 based upon sequence from Human
Host: QP13757 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of TK2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13757.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMVQRRA WPPDKEQEKE KKSVICVEGN IASGKTTCLE FFSNATDVEV LTEPVSKWRN VRGHNPLGLM YHDASRWGLT LQTYVQLTML DRHTRPQVSS VRLMERSIHS ARYIFVENLY RSGKMPEVDY VVLSEWFDWI LRNMDVSVDL IVYLRTNPET CYQRLKKRCR EEEKVIPLEY LEAIHHLHEE WLIKGSLFPM AAPVLVIEAD HHMERMLELF EQNRDRILTP ENRKHCP
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: TK2 protein solution (0.5 mg/ml) containing 20mM Tris-HCl buffer, pH 8.0, 30% glycerol, 2mM DTT and 200mM NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human TK2 General Information | |
|---|---|
| Alternate Names | |
| Thymidine kinase 2 mitochondrial, Mt-TK, TK2, MTTK, MTDPS2. | |
| Molecular Weight | |
| 30.2 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | O00142 |
| General Description of Recombinant Human TK2. | |
| Human TK2 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 257 amino acids (34-265 AA) and having a molecular mass of 30.2kDa. TK2 is fused to a 25 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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