PDF DatasheetDISC: Human TNNI2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant TNNI2 based upon sequence from Human
Host: QP13776 protein expressed in E. Coli.
Available Tags: His, Untagged
Protein Construction: A cDNA sequence encoding the sequence of TNNI2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13776.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGDEEKRNRA ITARRQHLKS VMLQIAATEL EKEESRREAE KQNYLAEHCP PLHIPGSMSE VQELCKQLHA KIDAAEEEKY DMEVRVQKTS KELEDMNQKL FDLRGKFKRP PLRRVRMSAD AMLKALLGSK HKVCMDLRAN LKQVKKEDTE KERDLRDVGD WRKNIEEKSG MEGRKKMFES ES
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The TNNI2 solution (0.25 mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 2M Urea, 20% glycerol and 0.2M NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human TNNI2 General Information | |
|---|---|
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| Alternate Names | |
| AMCD2B, DA2B, FSSV, fsTnI, Troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform. | |
| Molecular Weight | |
| 23.5 kDa | |
| Chromosomal Location | |
| p15.5 on chromosome 11 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TNNI2 |
| Entrez Gene ID | 7136 |
| UniProt ID(s) | P48788 |
| COSMIC ID Link(s) | TNNI2 |
| KEGG Gene ID(s) | hsa:7136 |
| General Description of Recombinant Human TNNI2. | |
| Human TNNI2 produced in E. coli is a single polypeptide chain containing 202 amino acids (1-182) and having a molecular mass of 23.5 kDa. TNNI2 is fused to 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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