PDF DatasheetHuman UBE2C Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant UBE2C based upon sequence from Human
Host: QP13854 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of UBE2C was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13854.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASQNRD PAATSVAAAR KGAEPSGGAA RGPVGKRLQQ ELMTLMMSGD KGISAFPESD NLFKWVGTIH GAAGTVYEDL RYKLSLEFPS GYPYNAPTVK FLTPCYHPNV DTQGNICLDI LKEKWSALYD VRTILLSIQS LLGEPNIDSP LNTHAAELWK NPTAFKKYLQ ETYSKQVTSQ EP
Purity: Greater than 90.0% as determined by Analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: UBE2C protein 1 mg/ml is supplied in 20mM Tris-HCL, pH 8.0, 0.15M NaCl, 1mM DTT and 10% Glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human UBE2C General Information | |
|---|---|
| Alternate Names | |
| Ubiquitin-conjugating enzyme E2 C, EC 6.3. 2.19, Ubiquitin-protein ligase C, Ubiquitin carrier protein C, Ubc10, UBCH10, dJ447F3.2. | |
| Molecular Weight | |
| 22.1 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | O00762 |
| General Description of Recombinant Human UBE2C. | |
| Human UBE2C produced in E. coli is a single, non-glycosylated polypeptide chain containing 202 amino acids (1-179) and having a molecular mass of 22.1 kDa. The UBE2C is fused to a 23 amino acid His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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