PDF Datasheetother UNG Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant UNG based upon sequence from other
Host: QP13896 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of UNG was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Reaction Conditions: 1X UNG Reaction Buffer, incubate at 37°C. UNG is active over a broad pH rabge with an optimum at pH 8.0. It doesn’t require a divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.
Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA.
Definition of Unit (for specific activity): 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C.
Bioactivity Data:
Specific Activity: The Specific Activity was found to be 5U/µl.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: UNG solution in 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.
Storage Conditions: Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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