PDF DatasheetE. Coli UNG Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant UNG based upon sequence from E. Coli
Host: QP13895 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of UNG was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13895.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: UNG protein solution (0.5 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant E. Coli UNG General Information | |
|---|---|
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| Alternate Names | |
| UDG, b2580, JW2564, EC 3.2. 2.27, DGU, UNG15, HIGM5, Uracil-DNA Glycosylase 1, EC 3.2. 2, HIGM4, UNG2. | |
| Molecular Weight | |
| 28.1 kDa | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ung |
| Entrez Gene ID | 947067 |
| UniProt ID(s) | P12295 |
| COSMIC ID Link(s) | ung |
| KEGG Gene ID(s) | ecj:JW2564; eco:b2580 |
| General Description of Recombinant E. Coli UNG. | |
| UNG E. Coli Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-229 AA) and having a molecular mass of 28.1kDa. UNG is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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