PDF DatasheetHuman UPRT Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant UPRT based upon sequence from Human
Host: QP13902 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of UPRT was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13902.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMATELQC PDSMPCHNQQ VNSASTPSPE QLRPGDLILD HAGGNRASRA KVILLTGYAH SSLPAELDSG ACGGSSLNSE GNSGSGDSSS YDAPAGNSFL EDCELSRQIG AQLKLLPMND QIRELQTIIR DKTASRGDFM FSADRLIRLV VEEGLNQLPY KECMVTTPTG YKYEGVKFEK GNCGVSIMRS GEAMEQGLRD CCRSIRIGKI LIQSDEETQR AKVYYAKFPP DIYRRKVLLM YPILSTGNTV IEAVKVLIEH GVQPSVIILL SLFSTPHGAK SIIQEFPEIT ILTTEVHPVA PTHFGQKYFG TD
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: UPRT protein solution (0.5 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human UPRT General Information | |
|---|---|
| Alternate Names | |
| Uracil phosphoribosyltransferase homolog, Uracil phosphoribosyltransferase (FUR1) homolog (S. cerevisiae), FUR1, Uracil Phosphoribosyltransferase, RP11-311P8.3, UPP, UPRT, UPRTase, UMP pyrophosphorylase, uprt. | |
| Molecular Weight | |
| 36.2 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q96BW1 |
| General Description of Recombinant Human UPRT. | |
| Human UPRT produced in E. Coli is a single, non-glycosylated polypeptide chain containing 332 amino acids (1-309 AA) and having a molecular mass of 36.2kDa. UPRT is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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