PDF DatasheetHuman VAPB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant VAPB based upon sequence from Human
Host: QP13922 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of VAPB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13922.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAKVEQVLSL EPQHELKFRG PFTDVVTTNL KLGNPTDRNV CFKVKTTAPR RYCVRPNSGI IDAGASINVS VMLQPFDYDP NEKSKHKFMV QSMFAPTDTS DMEAVWKEAK PEDLMDSKLR CVFELPAEND KPHDVEINKI ISTTASKTET PIVSKSLSSS LDDTEVKKVM EECKRLQGEV QRLREENKQF KEEDGLRMRK TVQSNSPISA LAPTGKEEGL ST
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The VAPB solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human VAPB General Information | |
|---|---|
| Alternate Names | |
| Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C, VAPB, ALS8, VAP-B, VAMP-B. | |
| Molecular Weight | |
| 27.1 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | O95292 |
| General Description of Recombinant Human VAPB. | |
| Human VAPB fused with a 20 amino acid His tag at N-terminus produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 242 amino acids (1-222 AA ) and having a molecular mass of 27.1kDa (molecular size on SDS-PAGE will appear higher). The VAPB is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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