PDF DatasheetHuman VAV1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant VAV1 based upon sequence from Human
Host: QP13927 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of VAV1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13927.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTEYDKR CCCLREIQQT EEKYTDTLGS IQQHFLKPLQ RFLKPQDIEI IFINIEDLLR VHTHFLKEMK EALGTPGAAN LYQVFIKYKE RFLVYGRYCS QVESASKHLD RVAAAREDVQ MKLEECSQRA NNGRFTLRDL LMVPMQRVLK YHLLLQELVK HTQEAMEKEN LRLALDAMRD LAQCVNEVKR DNETLRQITN FQLSIENLDQ SLAHYGRPKI DGELKITSVE RRSKMDRYAF LLDKALLICK RRGDSYDLKD FVNLHSFQVR DDSSGDRDNK KWSHMFLLIE DQGAQGYELF FKTRELKKKW MEQFEMAISN IYPENATANG HDFQMFSFEE TTSCKACQML LRGTFYQGYR CHRCRASAHK ECLGRVPPCG
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: VAV1 protein solution (0.25 mg/ml) containing In 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 30% glycerol and 2mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human VAV1 General Information | |
|---|---|
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| Alternate Names | |
| Vav 1 Guanine Nucleotide Exchange Factor, VAV, Vav 1 Oncogene, Proto-Oncogene Vav. | |
| Molecular Weight | |
| 46.8 kDa | |
| Chromosomal Location | |
| p13.3 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | VAV1 |
| Entrez Gene ID | 7409 |
| UniProt ID(s) | P15498 |
| COSMIC ID Link(s) | VAV1 |
| KEGG Gene ID(s) | hsa:7409 |
| General Description of Recombinant Human VAV1. | |
| Human VAV1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 400 amino acids (189-565AA) and having a molecular mass of 46.8kDa. VAV1 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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