PDF DatasheetHuman VPS25 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant VPS25 based upon sequence from Human
Host: QP13941 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of VPS25 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13941.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAMSFE WPWQYRFPPF FTLQPNVDTR QKQLAAWCSL VLSFCRLHKQ SSMTVMEAQE SPLFNNVKLQ RKLPVESIQI VLEELRKKGN LEWLDKSKSS FLIMWRRPEE WGKLIYQWVS RSGQNNSVFT LYELTNGEDT EDEEFHGLDE ATLLRALQAL QQEHKAEIIT VSDGRGVKFF
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The VPS25 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human VPS25 General Information | |
|---|---|
| Alternate Names | |
| Vacuolar protein sorting 25 homolog (S. cerevisiae), vacuolar protein-sorting-associated protein 25, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, Dermal papilla-derived protein 9, DERP9, EAP20, FAP20, MGC10540. | |
| Molecular Weight | |
| 23.3 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9BRG1 |
| General Description of Recombinant Human VPS25. | |
| Human VPS25 produced in E. coli is a single polypeptide chain containing 200 amino acids (1-176) and having a molecular mass of 23.3kDa. VPS25 is fused to a 24 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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