PDF DatasheetHuman VRK3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant VRK3 based upon sequence from Human
Host: QP13947 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of VRK3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13947.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMISFCPD CGKSIQAAFK FCPYCGNSLP VEEHVGSQTF VNPHVSSFQG SKRGLNSSFE TSPKKVKWSS TVTSPRLSLF SDGDSSESED TLSSSERSKG SGSRPPTPKS SPQKTRKSPQ VTRGSPQKTS CSPQKTRQSP QTLKRSRVTT SLEALPTGTV LTDKSGRQWK LKSFQTRDNQ GILYEAAPTS TLTCDSGPQK QKFSLKLDAK DGRLFNEQNF FQRAAKPLQV NKWKKLYSTP LLAIPTCMGF GVHQDKYRFL VLPSLGRSLQ SALDVSPKHV LSERSVLQVA CRLLDALEFL HENEYVHGNV TAENIFVDPE DQSQVTLAGY GFAFRYCPSG KHVAYVEGSR SPHEGDLEFI SMDLHKGCGP SRRSDLQSLG YCMLKWLYGF LPWTNCLPNT EDIMKQKQKF VDKPGPFVGP CGHWIRPSET LQKYLKVVMA LTYEEKPPYA MLRNNLEALL QDLRVSPYDP IGLPMVP
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The VRK3 solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 40% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human VRK3 General Information | |
|---|---|
| Alternate Names | |
| Serine/threonine-protein pseudokinase VRK3, vaccinia related kinase 3, inactive serine/threonine-protein kinase VRK3. | |
| Molecular Weight | |
| 55.3 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q8IV63 |
| General Description of Recombinant Human VRK3. | |
| Human VRK3 produced in E. coli is a single polypeptide chain containing 497 amino acids (1-474) and having a molecular mass of 55.3 kDa. VRK3 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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