PDF DatasheetHuman WIF1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant WIF1 based upon sequence from Human
Host: QP13959 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of WIF1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13959.
Bioactivity Data: Untested
Amino Acid Sequence: ADLGPPQEES LYLWIDAHQA RVLIGFEEDI LIVSEGKMAP FTHDFRKAQQ RMPAIPVNIH SMNFTWQAAG QAEYFYEFLS LRSLDKGIMA DPTVNVPLLG TVPHKASVVQ VGFPCLGKQD GVAAFEVDVI VMNSEGNTIL KTPQNAIFFK TCQQAECPGG CRNGGFCNER RICECPDGFH GPHCEKALCT PRCMNGGLCV TPGFCICPPG FYGVNCDKAN CSTTCFNGGT CFYPGKCICP PGLEGEQCEI SKCPQPCRNG GKCIGKSKCK CSKGYQGDLC SKPVCEPGCG AHGTCHEPNK CQCQEGWHGR HCNKRYEASL IHALRPAGAQ LRQHTPSLKK AEERRDPPES NYIWSGRLVP RGSHHHHHH
Purity: Greater than 80.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The WIF1 protein solution contains 1X PBS pH 7.4, 10% glycerol and 1mM PMSF.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human WIF1 General Information | |
|---|---|
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| Alternate Names | |
| WIF1, WIF-1, Wnt inhibitory factor 1. | |
| Molecular Weight | |
| 40.5 kDa | |
| Chromosomal Location | |
| q14.3 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | WIF1 |
| Entrez Gene ID | 11197 |
| UniProt ID(s) | Q9Y5W5 |
| COSMIC ID Link(s) | WIF1 |
| KEGG Gene ID(s) | hsa:11197 |
| General Description of Recombinant Human WIF1. | |
| WIF1 produced in Hi-5 cells is a single, glycosylated polypeptide chain containing 369 amino acids (29-379 AA ) and having a molecular mass of 40.5 kDa, (48kDa on SDS-PAGE). WIF1 is fused to 16 amino acid His Tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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