PDF DatasheetHuman WNT7A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant WNT7A based upon sequence from Human
Host: QP13963 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of WNT7A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13963.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSLGASIIC NKIPGLAPRQ RAICQSRPDA IIVIGEGSQM GLDECQFQFR NGRWNCSALG ERTVFGKELK VGSREAAFTY AIIAAGVAHA ITAACTQGNL SDCGCDKEKQ GQYHRDEGWK WGGCSADIRY GIGFAKVFVD AREIKQNART LMNLHNNEAG RKILEENMKL ECKCHGVSGS CTTKTCWTTL PQFRELGYVL KDKYNEAVHV EPVRASRNKR PTFLKIKKPL SYRKPMDTDL VYIEKSPNYC EEDPVTGSVG TQGRACNKTA PQASGCDLMC CGRGYNTHQY ARVWQCNCKF HWCCYVKCNT CSERTEMYTC K
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: WNT7A protein solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human WNT7A General Information | |
|---|---|
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| Alternate Names | |
| Wingless-Type MMTV Integration Site Family, Member 7A, Proto-Oncogene Wnt7a Protein, Protein Wnt-7a. | |
| Molecular Weight | |
| 38 kDa | |
| Chromosomal Location | |
| p25.1 on chromosome 3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | WNT7A |
| Entrez Gene ID | 7476 |
| UniProt ID(s) | O00755 |
| COSMIC ID Link(s) | WNT7A |
| KEGG Gene ID(s) | hsa:7476 |
| General Description of Recombinant Human WNT7A. | |
| Human WNT7A produced in E. Coli is a single, non-glycosylated polypeptide chain containing 341 amino acids (32-349 AA) and having a molecular mass of 38kDa. WNT7A is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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