PDF DatasheetHuman YARS2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant YARS2 based upon sequence from Human
Host: QP13981 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of YARS2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13981.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTLNLSVLLP LGLRKAHSGA QGLLAAQKAR GLFKDFFPET GTKIELPELF DRGTASFPQT IYCGFDPTAD SLHVGHLLAL LGLFHLQRAG HNVIALVGGA TARLGDPSGR TKEREALETE RVRANARALR LGLEALAANH QQLFTDGRSW GSFTVLDNSA WYQKQHLVDF LAAVGGHFRM GTLLSRQSVQ LRLKSPEGMS LAEFFYQVLQ AYDFYYLFQR YGCRVQLGGS DQLGNIMSGY EFINKLTGED VFGITVPLIT STTGAKLGKS AGNAVWLNRD KTSPFELYQF FVRQPDDSVE RYLKLFTFLP LPEIDHIMQL HVKEPERRGP QKRLAAEVTK LVHGREGLDS AKRCTQALYH SSIDALEVMS DQELKELFKE APFSEFFLDP GTSVLDTCRK ANAIPDGPRG YRMITEGGVS INHQQVTNPE SVLIVGQHIL KNGLSLLKIG KRNFYIIKWL QL
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: YARS2 protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1M NaCl, 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human YARS2 General Information | |
|---|---|
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| Alternate Names | |
| Tyrosine–tRNA ligase mitochondrial, Tyrosyl-tRNA synthetase, TyrRS, YARS2, CGI-04, TYRRS, MLASA2, MT-TYRRS. | |
| Molecular Weight | |
| 53.7 kDa | |
| Chromosomal Location | |
| p11.21 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | YARS2 |
| Entrez Gene ID | 51067 |
| UniProt ID(s) | Q9Y2Z4 |
| COSMIC ID Link(s) | YARS2 |
| KEGG Gene ID(s) | hsa:51067 |
| General Description of Recombinant Human YARS2. | |
| Human YARS2 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 482 amino acids (17-477 AA ) and having a molecular mass of 53.7kDa. YARS2 is fused to a 21 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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