PDF DatasheetMouse Phosphatidylcholine translocator ABCB4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Phosphatidylcholine translocator ABCB4 based upon sequence from Mouse
Host: QP5601 protein expressed in E.coli, Yeast..
Tag: GST
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) Phosphatidylcholine translocator ABCB4, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP5601.
Bioactivity Data: Untested
Full Length? Cytoplasmic Domain
Expression Region: Asp352 – Phe708
Amino Acid Sequence: DAFANARGAA YVIFDIIDNN PKIDSFSERG HKPDNIKGNL EFSDVHFSYP SRANIKILKG LNLKVKSGQT VALVGNSGCG KSTTVQLLQR LYDPTEGKIS IDGQDIRNFN VRCLREIIGV VSQEPVLFST TIAENIRYGR GNVTMDEIEK AVKEANAYDF IMKLPQKFDT LVGDRGAQLS GGQKQRIAIA RALVRNPKIL LLDEATSALD TESEAEVQAA LDKAREGRTT IVIAHRLSTI RNADVIAGFE DGVIVEQGSH SELMKKEGIY FRLVNMQTAG SQILSEEFEV ELSDEKAAGD VAPNGWKARI FRNSTKKSLK SPHQNRLDEE TNELDANVPP VSFLKVLKLN KTEWPYF
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Mouse Phosphatidylcholine translocator ABCB4 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse Phosphatidylcholine translocator ABCB4 Protein General Information | |
|---|---|
| Alternate Names | |
| Pgy2, Pgy-2, mdr-2, Mdr2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Abcb4 |
| Entrez Gene ID | 18670 |
| Ensemble Gene ID | ENSMUSG00000042476 |
| RefSeq Protein Accession(s) | NP_032856.2 |
| RefSeq mRNA Accession(s) | NM_008830.2 |
| UniProt ID(s) | P21440 |
| UniGene ID(s) | Mm.297825 |
| COSMIC ID Link(s) | Abcb4 |
| KEGG Gene ID(s) | mmu:18670 |
| General Description of Recombinant Mouse Phosphatidylcholine translocator ABCB4 Protein. | |
| Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi between hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:8106172, PubMed:7912658, PubMed:7592705, PubMed:7814632, PubMed:8725158, PubMed:9366571). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (By similarity). Required for proper phospholipid bile formation (PubMed:8106172). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed:7814632, PubMed:8725158). May promote biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance (PubMed:1990275). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.