PDF DatasheetHuman Apoptosis-inducing factor 1, mitochondrial Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Apoptosis-inducing factor 1, mitochondrial based upon sequence from Human
Host: QP5633 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Apoptosis-inducing factor 1, mitochondrial, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP5633.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Gly103 – Glu612
Amino Acid Sequence: GLTPEQKQKK AALSASEGEE VPQDKAPSHV PFLLIGGGTA AFAAARSIRA RDPGARVLIV SEDPELPYMR PPLSKELWFS DDPNVTKTLR FKQWNGKERS IYFQPPSFYV SAQDLPHIEN GGVAVLTGKK VVQLDVRDNM VKLNDGSQIT YEKCLIATGG TPRSLSAIDR AGAEVKSRTT LFRKIGDFRS LEKISREVKS ITIIGGGFLG SELACALGRK ARALGTEVIQ LFPEKGNMGK ILPEYLSNWT MEKVRREGVK VMPNAIVQSV GVSSGKLLIK LKDGRKVETD HIVAAVGLEP NVELAKTGGL EIDSDFGGFR VNAELQARSN IWVAGDAACF YDIKLGRRRV EHHDHAVVSG RLAGENMTGA AKPYWHQSMF WSDLGPDVGY EAIGLVDSSL PTVGVFAKAT AQDNPKSATE QSGTGIRSES ETESEASEIT IPPSTPAVPQ APVQGEDYGK GVIFYLRDKV VVGIVLWNIF NRMPIARKII KDGEQHEDLN EVAKLFNIHE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Apoptosis-inducing factor 1, mitochondrial Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Apoptosis-inducing factor 1, mitochondrial Protein General Information | |
|---|---|
| Alternate Names | |
| COXPD6, DFNX5, PDCD8, NAMSD, AIF, COWCK, CMT2D, CMTX4, NADMR | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | AIFM1 |
| Entrez Gene ID | 9131 |
| Ensemble Gene ID | ENSG00000156709 |
| RefSeq Protein Accession(s) | NP_001124318.2 |
| RefSeq mRNA Accession(s) | NM_001130846.3, NM_001130847.3, NM_004208.3, NM_145812.2 |
| UniProt ID(s) | O95831 |
| UniGene ID(s) | Hs.424932 |
| HGNC ID(s) | HGNC:8768 |
| COSMIC ID Link(s) | AIFM1 |
| KEGG Gene ID(s) | hsa:9131 |
| PharmGKB ID(s) | PA162376129 |
| General Description of Recombinant Human Apoptosis-inducing factor 1, mitochondrial Protein. | |
| Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermbrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces ‘parthanatos’ i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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