PDF DatasheetHuman Ubiquitin-like-conjugating enzyme ATG3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Ubiquitin-like-conjugating enzyme ATG3 based upon sequence from Human
Host: QP5690 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Ubiquitin-like-conjugating enzyme ATG3, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP5690.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Met314
Amino Acid Sequence: MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT YHNTGITGIT EAVKEITLEN KDNIRLQDCS ALCEEEEDED EGEAADMEEY EESGLLETDE ATLDTRKIVE ACKAKTDAGG EDAILQTRTY DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH VKKTVTIENH PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV IPTIEYDYTR HFTM
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Ubiquitin-like-conjugating enzyme ATG3 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Ubiquitin-like-conjugating enzyme ATG3 Protein General Information | |
|---|---|
| Alternate Names | |
| APG3L, PC3-96, APG3, APG3-LIKE | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ATG3 |
| Entrez Gene ID | 64422 |
| Ensemble Gene ID | ENSG00000144848 |
| RefSeq Protein Accession(s) | NP_001265641.1 |
| RefSeq mRNA Accession(s) | NM_001278712.1, NM_022488.4 |
| UniProt ID(s) | Q9NT62 |
| UniGene ID(s) | Hs.477126 |
| HGNC ID(s) | HGNC:20962 |
| COSMIC ID Link(s) | ATG3 |
| KEGG Gene ID(s) | hsa:64422 |
| PharmGKB ID(s) | PA134883444 |
| General Description of Recombinant Human Ubiquitin-like-conjugating enzyme ATG3 Protein. | |
| E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the mbrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by roving OFD1 from centriolar satellites via the autophagic pathway. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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