PDF DatasheetMouse Beclin-1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Beclin-1 based upon sequence from Mouse
Host: QP5715 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) Beclin-1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP5715.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Lys448
Amino Acid Sequence: MEGSKASSST MQVSFVCQRC SQPLKLDTSF KILDRVTIQE LTAPLLTTAQ AKPGETQEEE ANSGEEPFIE TRQDGVSRRF IPPARMMSTE SANSFTLIGE ASDGGTMENL SRRLKVTGDL FDIMSGQTDV DHPLCEECTD TLLDQLDTQL NVTENECQNY KRCLEILEQM NEDDSEQLQR ELKELALEEE RLIQELEDVE KNRKVVAENL EKVQAEAERL DQEEAQYQRE YSEFKRQQLE LDDELKSVEN QVRYAQIQLD KLKKTNVFNA TFHIWHSGQF GTINNFRLGR LPSVPVEWNE INAAWGQTVL LLHALANKMG LKFQRYRLVP YGNHSYLESL TDKSKELPLY CSGGLRFFWD NKFDHAMVAF LDCVQQFKEE VEKGETRFCL PYRMDVEKGK IEDTGGSGGS YSIKTQFNSE EQWTKALKFM LTNLKWGLAW VSSQFYNK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Mouse Beclin-1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse Beclin-1 Protein General Information | |
|---|---|
| Alternate Names | |
| 4921513J16Rik, Atg6, 5430417M23Rik | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Becn1 |
| Entrez Gene ID | 56208 |
| Ensemble Gene ID | ENSMUSG00000035086 |
| RefSeq Protein Accession(s) | NP_062530.2 |
| RefSeq mRNA Accession(s) | NM_019584.3 |
| UniProt ID(s) | O88597 |
| UniGene ID(s) | Mm.178947 |
| COSMIC ID Link(s) | Becn1 |
| KEGG Gene ID(s) | mmu:56208 |
| General Description of Recombinant Mouse Beclin-1 Protein. | |
| Plays a central role in autophagy (PubMed:10604474, PubMed:12372286, PubMed:19270693). Acts as core subunit of different PI3K complex forms that mediate formation of phosphatidylinositol 3-phosphate and are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed:19270693, PubMed:25275521). Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (By similarity). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms (PubMed:25275521). Involved in endocytosis including endosome formation in neuronal cells (PubMed:25275521). May play a role in antiviral host defense (By similarity). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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