PDF DatasheetHuman Lys-63-specific deubiquitinase BRCC36 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Lys-63-specific deubiquitinase BRCC36 based upon sequence from Human
Host: QP5727 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Lys-63-specific deubiquitinase BRCC36, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP5727.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Glu316
Amino Acid Sequence: AVQVVQAVQA VHLESDAFLV CLNHALSTEK EEVMGLCIGE LNDDTRSDSK FAYTGTEMRT VAEKVDAVRI VHIHSVIILR RSDKRKDRVE ISPEQLSAAS TEAERLAELT GRPMRVVGWY HSHPHITVWP SHVDVRTQAM YQMMDQGFVG LIFSCFIEDK NTKTGRVLYT CFQSIQAQKS SESLHGPRDF WSSSQHISIE GQKEEERYER IEIPIHIVPH VTIGKVCLES AVELPKILCQ EEQDAYRRIH SLTHLDSVTK IHNGSVFTKN LCSQMSAVSG PLLQWLEDRL EQNQQHLQEL QQEKEELMQE LSSLE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Lys-63-specific deubiquitinase BRCC36 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Lys-63-specific deubiquitinase BRCC36 Protein General Information | |
|---|---|
| Alternate Names | |
| C6.1A, BRCC36, CXorf53 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | BRCC3 |
| Entrez Gene ID | 79184 |
| Ensemble Gene ID | ENSG00000185515 |
| RefSeq Protein Accession(s) | NP_001018065.1 |
| RefSeq mRNA Accession(s) | NM_001018055.2, NM_001242640.1, NM_024332.3, XM_017029838.1 |
| UniProt ID(s) | P46736 |
| UniGene ID(s) | Hs.558537 |
| HGNC ID(s) | HGNC:24185 |
| COSMIC ID Link(s) | BRCC3 |
| KEGG Gene ID(s) | hsa:79184 |
| PharmGKB ID(s) | PA134922847 |
| General Description of Recombinant Human Lys-63-specific deubiquitinase BRCC36 Protein. | |
| Metalloprotease that specifically cleaves ‘Lys-63’-linked polyubiquitin chains. Does not have activity toward ‘Lys-48’-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes ‘Lys-63’-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically roves ‘Lys-63’-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves ‘Lys-63’-linked ubiquitin in various substrates. Mediates the specific ‘Lys-63’-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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