PDF DatasheetHuman Cyclin D1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Cyclin D1 based upon sequence from Human
Host: QP5776 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Cyclin D1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP5776.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Ile295
Amino Acid Sequence: MEHQLLCCEV ETIRRAYPDA NLLNDRVLRA MLKAEETCAP SVSYFKCVQK EVLPSMRKIV ATWMLEVCEE QKCEEEVFPL AMNYLDRFLS LEPVKKSRLQ LLGATCMFVA SKMKETIPLT AEKLCIYTDN SIRPEELLQM ELLLVNKLKW NLAAMTPHDF IEHFLSKMPE AEENKQIIRK HAQTFVALCA TDVKFISNPP SMVAAGSVVA AVQGLNLRSP NNFLSYYRLT RFLSRVIKCD PDCLRACQEQ IEALLESSLR QAQQNMDPKA AEEEEEEEEE VDLACTPTDV RDVDI
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Cyclin D1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Cyclin D1 Protein General Information | |
|---|---|
| Alternate Names | |
| Cyclin D1, D11S287E, BCL1, U21B31, PRAD1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CCND1 |
| Entrez Gene ID | 595 |
| Ensemble Gene ID | ENSG00000110092 |
| RefSeq Protein Accession(s) | NP_444284.1 |
| RefSeq mRNA Accession(s) | NM_053056.2 |
| UniProt ID(s) | P24385 |
| UniGene ID(s) | Hs.523852 |
| HGNC ID(s) | HGNC:1582 |
| COSMIC ID Link(s) | CCND1 |
| KEGG Gene ID(s) | hsa:595 |
| PharmGKB ID(s) | PA75 |
| General Description of Recombinant Human Cyclin D1 Protein. | |
| Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits mbers of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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