PDF DatasheetHuman Egl nine homolog 2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Egl nine homolog 2 based upon sequence from Human
Host: QP5968 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Egl nine homolog 2, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP5968.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Met283 – Thr407
Amino Acid Sequence: MVACYPGNGL GYVRHVDNPH GDGRCITCIY YLNQNWDVKV HGGLLQIFPE GRPVVANIEP LFDRLLIFWS DRRNPHEVKP AYATRYAITV WYFDAKERAA AKDKYQLASG QKGVQVPVSQ PPTPT
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Egl nine homolog 2 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Egl nine homolog 2 Protein General Information | |
|---|---|
| Alternate Names | |
| HIF-PH1, EIT6, HIFPH1, PHD1, HPH-1, HPH-3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EGLN2 |
| Entrez Gene ID | 112398 |
| Ensemble Gene ID | ENSG00000269858 |
| RefSeq Protein Accession(s) | NP_444274.1 |
| RefSeq mRNA Accession(s) | NM_053046.3, NM_080732.3 |
| UniProt ID(s) | Q96KS0 |
| UniGene ID(s) | Hs.515417 |
| HGNC ID(s) | HGNC:14660 |
| COSMIC ID Link(s) | EGLN2 |
| KEGG Gene ID(s) | hsa:112398 |
| PharmGKB ID(s) | PA27671 |
| General Description of Recombinant Human Egl nine homolog 2 Protein. | |
| Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferencially recognized via a LXXLAP motif. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.