PDF DatasheetHuman EIF2AK2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant EIF2AK2 based upon sequence from Human
Host: QP5969 protein expressed in E.coli, Yeast..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) EIF2AK2, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP5969.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Cys551
Amino Acid Sequence: AGDLSAGFFM EELNTYRQKQ GVVLKYQELP NSGPPHDRRF TFQVIIDGRE FPEGEGRSKK EAKNAAAKLA VEILNKEKKA VSPLLLTTTN SSEGLSMGNY IGLINRIAQK KRLTVNYEQC ASGVHGPEGF HYKCKMGQKE YSIGTGSTKQ EAKQLAAKLA YLQILSEETS VKSDYLSSGS FATTCESQSN SLVTSTLASE SSSEGDFSAD TSEINSNSDS LNSSSLLMNG LRNNQRKAKR SLAPRFDLPD MKETKYTVDK RFGMDFKEIE LIGSGGFGQV FKAKHRIDGK TYVIKRVKYN NEKAEREVKA LAKLDHVNIV HYNGCWDGFD YDPETSDDSL ESSDYDPENS KNSSRSKTKC LFIQMEFCDK GTLEQWIEKR RGEKLDKVLA LELFEQITKG VDYIHSKKLI HRDLKPSNIF LVDTKQVKIG DFGLVTSLKN DGKRTRSKGT LRYMSPEQIS SQDYGKEVDL YALGLILAEL LHVCDTAFET SKFFTDLRDG IISDIFDKKE KTLLQKLLSK KPEDRPNTSE ILRTLTVWKK SPEKNERHTC
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human EIF2AK2 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human EIF2AK2 Protein General Information | |
|---|---|
| Alternate Names | |
| PKR, EIF2AK1, PPP1R83, PRKR, Interferon-induced, double-stranded RNA-activated protein kina | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EIF2AK2 |
| Entrez Gene ID | 5610 |
| Ensemble Gene ID | ENSG00000055332 |
| RefSeq Protein Accession(s) | NP_001129123.1 |
| RefSeq mRNA Accession(s) | NM_001135651.2, NM_001135652.2, NM_002759.3, XM_011532987.2 |
| UniProt ID(s) | P19525 |
| UniGene ID(s) | Hs.131431 |
| HGNC ID(s) | HGNC:9437 |
| COSMIC ID Link(s) | EIF2AK2 |
| KEGG Gene ID(s) | hsa:5610 |
| PharmGKB ID(s) | PA33779 |
| General Description of Recombinant Human EIF2AK2 Protein. | |
| IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at ‘Tyr-4’ upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at ‘Ser-312’ and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assbly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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