PDF DatasheetHuman EIF3E Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant EIF3E based upon sequence from Human
Host: QP5971 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) EIF3E, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP5971.
Bioactivity Data: Untested
Full Length? Full Length
Amino Acid Sequence: AEYDLTTRIA HFLDRHLVFP LLEFLSVKEI YNEKELLQGK LDLLSDTNMV DFAMDVYKNL YSDDIPHALR EKRTTVVAQL KQLQAETEPI VKMFEDPETT RQMQSTRDGR MLFDYLADKH GFRQEYLDTL YRYAKFQYEC GNYSGAAEYL YFFRVLVPAT DRNALSSLWG KLASEILMQN WDAAMEDLTR LKETIDNNSV SSPLQSLQQR TWLIHWSLFV FFNHPKGRDN IIDLFLYQPQ YLNAIQTMCP HILRYLTTAV ITNKDVRKRR QVLKDLVKVI QQESYTYKDP ITEFVECLYV NFDFDGAQKK LRECESVLVN DFFLVACLED FIENARLFIF ETFCRIHQCI SINMLADKLN MTPEEAERWI VNLIRNARLD AKIDSKLGHV VMGNNAVSPY QQVIEKTKSL SFRSQMLAMN IEKKLNQNSR SEAPNWATQD SGFY
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human EIF3E Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human EIF3E Protein General Information | |
|---|---|
| Alternate Names | |
| INT6, EIF3-P48, eIF3-p46, EIF3S6, Eukaryotic translation initiation factor 3 subunit | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EIF3E |
| Entrez Gene ID | 3646 |
| Ensemble Gene ID | ENSG00000104408 |
| RefSeq Protein Accession(s) | NP_001559.1 |
| RefSeq mRNA Accession(s) | NM_001568.2 |
| UniProt ID(s) | P60228 |
| UniGene ID(s) | Hs.405590 |
| HGNC ID(s) | HGNC:3277 |
| COSMIC ID Link(s) | EIF3E |
| KEGG Gene ID(s) | hsa:3646 |
| PharmGKB ID(s) | PA27705 |
| General Description of Recombinant Human EIF3E Protein. | |
| omponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis . The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation . The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression . Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway . May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.