PDF DatasheetHuman Histone H3.3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Histone H3.3 based upon sequence from Human
Host: QP6141 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Histone H3.3, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6141.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Ala136
Amino Acid Sequence: ARTKQTARKS TGGKAPRKQL ATKAARKSAP STGGVKKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSAAIG ALQEASEAYL VGLFEDTNLC AIHAKRVTIM PKDIQLARRI RGERA
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Histone H3.3 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Histone H3.3 Protein General Information | |
|---|---|
| Alternate Names | |
| H3.3A, H3F3, H3.3B | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | H3F3A |
| Entrez Gene ID | 3020 |
| Ensemble Gene ID | ENSG00000132475 |
| RefSeq Protein Accession(s) | NP_002098.1 |
| RefSeq mRNA Accession(s) | NM_002107.4, NM_005324.4 |
| UniProt ID(s) | P84243 |
| UniGene ID(s) | Hs.180877 |
| HGNC ID(s) | HGNC:4764 |
| COSMIC ID Link(s) | H3F3A |
| KEGG Gene ID(s) | hsa:3020 |
| PharmGKB ID(s) | PA29140 |
| General Description of Recombinant Human Histone H3.3 Protein. | |
| Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacent throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a tplate. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome rodeling. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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