PDF DatasheetHuman HDAC3 / Histone deacetylase 3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HDAC3 / Histone deacetylase 3 based upon sequence from Human
Host: QP6152 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) HDAC3 / Histone deacetylase 3, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6152.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Ile428
Amino Acid Sequence: MAKTVAYFYD PDVGNFHYGA GHPMKPHRLA LTHSLVLHYG LYKKMIVFKP YQASQHDMCR FHSEDYIDFL QRVSPTNMQG FTKSLNAFNV GDDCPVFPGL FEFCSRYTGA SLQGATQLNN KICDIAINWA GGLHHAKKFE ASGFCYVNDI VIGILELLKY HPRVLYIDID IHHGDGVQEA FYLTDRVMTV SFHKYGNYFF PGTGDMYEVG AESGRYYCLN VPLRDGIDDQ SYKHLFQPVI NQVVDFYQPT CIVLQCGADS LGCDRLGCFN LSIRGHGECV EYVKSFNIPL LVLGGGGYTV RNVARCWTYE TSLLVEEAIS EELPYSEYFE YFAPDFTLHP DVSTRIENQN SRQYLDQIRQ TIFENLKMLN HAPSVQIHDV PADLLTYDRT DEADAEERGP EENYSRPEAP NEFYDGDHDN DKESDVEI
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human HDAC3 / Histone deacetylase 3 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human HDAC3 / Histone deacetylase 3 Protein General Information | |
|---|---|
| Alternate Names | |
| HDAC3, Histone deacetylase 3, HD3, RPD3-2, RPD3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HDAC3 |
| Entrez Gene ID | 8841 |
| Ensemble Gene ID | ENSG00000171720 |
| RefSeq Protein Accession(s) | NP_003874.2 |
| RefSeq mRNA Accession(s) | NM_003883.3 |
| UniProt ID(s) | O15379 |
| UniGene ID(s) | Hs.519632 |
| HGNC ID(s) | HGNC:4854 |
| COSMIC ID Link(s) | HDAC3 |
| KEGG Gene ID(s) | hsa:8841 |
| PharmGKB ID(s) | PA29228 |
| General Description of Recombinant Human HDAC3 / Histone deacetylase 3 Protein. | |
| Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 ‘Lys-27’ (H3K27) on enhancer elents, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation . Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI3K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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