PDF DatasheetHuman Checkpoint protein HUS1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Checkpoint protein HUS1 based upon sequence from Human
Host: QP6186 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Checkpoint protein HUS1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6186.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Lys2 – Ser280
Amino Acid Sequence: KFRAKIVDGA CLNHFTRISN MIAKLAKTCT LRISPDKLNF ILCDKLANGG VSMWCELEQE NFFNEFQMEG VSAENNEIYL ELTSENLSRA LKTAQNARAL KIKLTNKHFP CLTVSVELLS MSSSSRIVTH DIPIKVIPRK LWKDLQEPVV PDPDVSIYLP VLKTMKSVVE KMKNISNHLV IEANLDGELN LKIETELVCV TTHFKDLGNP PLASESTHED RNVEHMAEVH IDIRKLLQFL AGQQVNPTKA LCNIVNNKMV HFDLLHEDVS LQYFIPALS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Checkpoint protein HUS1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Checkpoint protein HUS1 Protein General Information | |
|---|---|
| Alternate Names | |
| hHUS1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HUS1 |
| Entrez Gene ID | 3364 |
| Ensemble Gene ID | ENSG00000136273 |
| RefSeq Protein Accession(s) | NP_004498.1 |
| RefSeq mRNA Accession(s) | NM_004507.3, XM_017012115.1 |
| UniProt ID(s) | O60921 |
| UniGene ID(s) | Hs.152983 |
| HGNC ID(s) | HGNC:5309 |
| COSMIC ID Link(s) | HUS1 |
| KEGG Gene ID(s) | hsa:3364 |
| PharmGKB ID(s) | PA29564 |
| General Description of Recombinant Human Checkpoint protein HUS1 Protein. | |
| Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3′-OH end of the primer-tplate and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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