PDF DatasheetHuman IFIT3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant IFIT3 based upon sequence from Human
Host: QP6198 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) IFIT3, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6198.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Asn490
Amino Acid Sequence: MSEVTKNSLE KILPQLKCHF TWNLFKEDSV SRDLEDRVCN QIEFLNTEFK ATMYNLLAYI KHLDGNNEAA LECLRQAEEL IQQEHADQAE IRSLVTWGNY AWVYYHLGRL SDAQIYVDKV KQTCKKFSNP YSIEYSELDC EEGWTQLKCG RNERAKVCFE KALEEKPNNP EFSSGLAIAM YHLDNHPEKQ FSTDVLKQAI ELSPDNQYVK VLLGLKLQKM NKEAEGEQFV EEALEKSPCQ TDVLRSAAKF YRRKGDLDKA IELFQRVLES TPNNGYLYHQ IGCCYKAKVR QMQNTGESEA SGNKEMIEAL KQYAMDYSNK ALEKGLNPLN AYSDLAEFLE TECYQTPFNK EVPDAEKQQS HQRYCNLQKY NGKSEDTAVQ HGLEGLSISK KSTDKEEIKD QPQNVSENLL PQNAPNYWYL QGLIHKQNGD LLQAAKCYEK ELGRLLRDAP SGIGSIFLSA SELEDGSEEM GQGAVSSSPR ELLSNSEQLN
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human IFIT3 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human IFIT3 Protein General Information | |
|---|---|
| Alternate Names | |
| ISG60, CIG-49, IFI60, GARG-49, IFIT4, P60, RIG-G, IRG2, cig41, Interferon-induced protein with tetratricopeptide repeats | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IFIT3 |
| Entrez Gene ID | 3437 |
| Ensemble Gene ID | ENSG00000119917 |
| RefSeq Protein Accession(s) | NP_001026853.1 |
| RefSeq mRNA Accession(s) | NM_001031683.3, NM_001549.5 |
| UniProt ID(s) | O14879 |
| UniGene ID(s) | Hs.47338 |
| HGNC ID(s) | HGNC:5411 |
| COSMIC ID Link(s) | IFIT3 |
| KEGG Gene ID(s) | hsa:3437 |
| PharmGKB ID(s) | PA29651 |
| General Description of Recombinant Human IFIT3 Protein. | |
| IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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