PDF DatasheetHuman IGF1R / CD221 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant IGF1R / CD221 based upon sequence from Human
Host: QP6207 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) IGF1R / CD221, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6207.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Tyr763 – Glu931
Amino Acid Sequence: YNITDPEELE TEYPFFESRV DNKERTVISN LRPFTLYRID IHSCNHEAEK LGCSASNFVF ARTMPAEGAD DIPGPVTWEP RPENSIFLKW PEPENPNGLI LMYEIKYGSQ VEDQRECVSR QEYRKYGGAK LNRLNPGNYT ARIQATSLSG NGSWTDPVFF YVQAKTGYE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human IGF1R / CD221 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human IGF1R / CD221 Protein General Information | |
|---|---|
| Alternate Names | |
| IGF1R, CD221, JTK13, CD221, IGFIR, IGFR | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IGF1R |
| Entrez Gene ID | 3480 |
| Ensemble Gene ID | ENSG00000140443 |
| RefSeq Protein Accession(s) | NP_000866.1 |
| RefSeq mRNA Accession(s) | NM_000875.4, NM_001291858.1 |
| UniProt ID(s) | P08069 |
| UniGene ID(s) | Hs.643120 |
| HGNC ID(s) | HGNC:5465 |
| COSMIC ID Link(s) | IGF1R |
| KEGG Gene ID(s) | hsa:3480 |
| PharmGKB ID(s) | PA29698 |
| General Description of Recombinant Human IGF1R / CD221 Protein. | |
| Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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