PDF DatasheetHuman Tyrosine-protein kinase JAK2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Tyrosine-protein kinase JAK2 based upon sequence from Human
Host: QP6246 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Tyrosine-protein kinase JAK2, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6246.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Lys752 – Gly1132
Amino Acid Sequence: KPLSALDSQR KLQFYEDRHQ LPAPKWAELA NLINNCMDYE PDFRPSFRAI IRDLNSLFTP DYELLTENDM LPNMRIGALG FSGAFEDRDP TQFEERHLKF LQQLGKGNFG SVEMCRYDPL QDNTGEVVAV KKLQHSTEEH LRDFEREIEI LKSLQHDNIV KYKGVCYSAG RRNLKLIMEY LPYGSLRDYL QKHKERIDHI KLLQYTSQIC KGMEYLGTKR YIHRDLATRN ILVENENRVK IGDFGLTKVL PQDKEYYKVK EPGESPIFWY APESLTESKF SVASDVWSFG VVLYELFTYI EKSKSPPAEF MRMIGNDKQG QMIVFHLIEL LKNNGRLPRP DGCPDEIYMI MTECWNNNVN QRPSFRDLAL RVDQIRDNMA G
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Tyrosine-protein kinase JAK2 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Tyrosine-protein kinase JAK2 Protein General Information | |
|---|---|
| Alternate Names | |
| JTK10, THCYT3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | JAK2 |
| Entrez Gene ID | 3717 |
| Ensemble Gene ID | ENSG00000096968 |
| RefSeq Protein Accession(s) | NP_001309123.1 |
| RefSeq mRNA Accession(s) | NM_001322194.1, NM_001322195.1, NM_001322196.1, NM_001322204.1, NM_004972.3 |
| UniProt ID(s) | O60674 |
| UniGene ID(s) | Hs.656213 |
| HGNC ID(s) | HGNC:6192 |
| COSMIC ID Link(s) | JAK2 |
| KEGG Gene ID(s) | hsa:3717 |
| PharmGKB ID(s) | PA29989 |
| General Description of Recombinant Human Tyrosine-protein kinase JAK2 Protein. | |
| Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the Cytoplasmic domain tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its Cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of ‘Tyr-41’ of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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