PDF DatasheetHuman Importin subunit alpha-1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Importin subunit alpha-1 based upon sequence from Human
Host: QP6273 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Importin subunit alpha-1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6273.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Asn8 – Phe538
Amino Acid Sequence: NTPAARLHRF KNKGKDSTEM RRRRIEVNVE LRKAKKDDQM LKRRNVSSFP DDATSPLQEN RNNQGTVNWS VDDIVKGINS SNVENQLQAT QAARKLLSRE KQPPIDNIIR AGLIPKFVSF LGRTDCSPIQ FESAWALTNI ASGTSEQTKA VVDGGAIPAF ISLLASPHAH ISEQAVWALG NIAGDGSVFR DLVIKYGAVD PLLALLAVPD MSSLACGYLR NLTWTLSNLC RNKNPAPPID AVEQILPTLV RLLHHDDPEV LADTCWAISY LTDGPNERIG MVVKTGVVPQ LVKLLGASEL PIVTPALRAI GNIVTGTDEQ TQVVIDAGAL AVFPSLLTNP KTNIQKEATW TMSNITAGRQ DQIQQVVNHG LVPFLVSVLS KADFKTQKEA VWAVTNYTSG GTVEQIVYLV HCGIIEPLMN LLTAKDTKII LVILDAISNI FQAAEKLGET EKLSIMIEEC GGLDKIEALQ NHENESVYKA SLSLIEKYFS VEEEEDQNVV PETTSEGYTF QVQDGAPGTF NF
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Importin subunit alpha-1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Importin subunit alpha-1 Protein General Information | |
|---|---|
| Alternate Names | |
| IPOA1, QIP2, SRP1-alpha, SRP1alpha, RCH1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KPNA1 |
| Entrez Gene ID | 3838 |
| Ensemble Gene ID | ENSG00000182481 |
| RefSeq Protein Accession(s) | NP_001307540.1 |
| RefSeq mRNA Accession(s) | NM_001320611.1, NM_002266.3 |
| UniProt ID(s) | P52292 |
| UniGene ID(s) | Hs.594238 |
| HGNC ID(s) | HGNC:6395 |
| COSMIC ID Link(s) | KPNA1 |
| KEGG Gene ID(s) | hsa:3838 |
| PharmGKB ID(s) | PA30186 |
| General Description of Recombinant Human Importin subunit alpha-1 Protein. | |
| Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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