PDF DatasheetHuman MAGOH Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MAGOH based upon sequence from Human
Host: QP6333 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) MAGOH, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6333.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Ile146
Amino Acid Sequence: MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPI
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human MAGOH Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human MAGOH Protein General Information | |
|---|---|
| Alternate Names | |
| MAGOH, MAGOH1, MAGOHA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MAGOH |
| Entrez Gene ID | 4116 |
| Ensemble Gene ID | ENSG00000162385 |
| RefSeq Protein Accession(s) | NP_002361.1 |
| RefSeq mRNA Accession(s) | NM_002370.3 |
| UniProt ID(s) | P61326 |
| UniGene ID(s) | Hs.421576 |
| HGNC ID(s) | HGNC:6815 |
| COSMIC ID Link(s) | MAGOH |
| KEGG Gene ID(s) | hsa:4116 |
| PharmGKB ID(s) | PA30563 |
| General Description of Recombinant Human MAGOH Protein. | |
| Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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